Learning and Imputation for Mass-spec Bias Reduction (LIMBR).

ABSTRACT:

Motivation

Decreasing costs are making it feasible to perform time series proteomics and genomics experiments with more replicates and higher resolution than ever before. With more replicates and time points, proteome and genome-wide patterns of expression are more readily discernible. These larger experiments require more batches exacerbating batch effects and increasing the number of bias trends. In the case of proteomics, where methods frequently result in missing data this increasing scale is also decreasing the number of peptides observed in all samples. The sources of batch effects and missing data are incompletely understood necessitating novel techniques.

Results

Here we show that by exploiting the structure of time series experiments, it is possible to accurately an

SUBMITTER: Crowell AM 

PROVIDER: S-EPMC6499252 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature