Dataset Information

Pseudo-allergic reaction caused by Qingkailing injection partially via the PI3K-Rac1 signaling pathway in RBL-2H3 cells.

ABSTRACT: Qingkailing injection (QKLI) is a kind of multi-component traditional Chinese medicine injection. It has been widely used in clinical practice, but in recent years, it has caused more and more adverse reactions, mainly manifested as pseudo-allergic symptoms. To explore the potential mechanism of the pseudo-allergic reaction by QKLI, basophilic leukemia cell line 2H3 (RBL-2H3) was chosen. The results showed that QKLI at doses of 5, 10 and 20 mL L^-1 activated phosphoinositide 3-kinase (PI3K) activity and also increased the levels of Ras-related C3 botulinum toxin substrate 1 (Rac1), p21 protein-activated kinase 1 (Pak1), LIM kinase (Limk1) and cofilin (an actin polymerization regulator) proteins. What's more, QKLI aggravated the depolymerization of F-actin. NSC23766, a Rac1 inhibitor, reversed the previous results in QKLI-treated RBL-2H3 cells. In addition, when the Rac1 gene was knocked down using lentiviral vector-loaded shRNA in RBL-2H3 cells, the PI3K activity and depolymerization of F-actin were downregulated, hinting that the pseudo-allergic reaction was significantly reduced. In general, the pseudo-allergic reaction induced by QKLI was likely to be based on PI3K-Rac1 signaling pathways partially.

SUBMITTER: Li Q

PROVIDER: S-EPMC6505390 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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Pseudo-allergic reaction caused by Qingkailing injection partially <i>via</i> the PI3K-Rac1 signaling pathway in RBL-2H3 cells.

Li Qin Q Zhang Lingxi L Gu Lili L Zhang Bo B Lu Jiaqi J Zhang Xinyue X

Toxicology research 20190218 3

Qingkailing injection (QKLI) is a kind of multi-component traditional Chinese medicine injection. It has been widely used in clinical practice, but in recent years, it has caused more and more adverse reactions, mainly manifested as pseudo-allergic symptoms. To explore the potential mechanism of the pseudo-allergic reaction by QKLI, basophilic leukemia cell line 2H3 (RBL-2H3) was chosen. The results showed that QKLI at doses of 5, 10 and 20 mL L<sup>-1</sup> activated phosphoinositide 3-kinase ( ...[more]

PMID: 31160969

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Dataset Information

Pseudo-allergic reaction caused by Qingkailing injection partially via the PI3K-Rac1 signaling pathway in RBL-2H3 cells.

Publications

Pseudo-allergic reaction caused by Qingkailing injection partially <i>via</i> the PI3K-Rac1 signaling pathway in RBL-2H3 cells.

Similar Datasets

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