Ontology highlight
ABSTRACT:
SUBMITTER: Afshar Bakooshli M
PROVIDER: S-EPMC6516829 | biostudies-literature | 2019 May
REPOSITORIES: biostudies-literature
eLife 20190514
Two-dimensional (2D) human skeletal muscle fiber cultures are ill-equipped to support the contractile properties of maturing muscle fibers. This limits their application to the study of adult human neuromuscular junction (NMJ) development, a process requiring maturation of muscle fibers in the presence of motor neuron endplates. Here we describe a three-dimensional (3D) co-culture method whereby human muscle progenitors mixed with human pluripotent stem cell-derived motor neurons self-organize t ...[more]