Dataset Information

"Enhanced acquisition of antibiotic-resistant intestinal E. coli during the first year of life assessed in a prospective cohort study".

ABSTRACT:

Background

Increasing bacterial resistance to antibiotics is a serious problem worldwide. We sought to record the acquisition of antibiotic-resistant Escherichia coli (E. coli) in healthy infants in Northern Thailand and investigated potential determinants.

Methods

Stool samples from 142 infants after birth, at ages 2wk, 2mo, 4 to 6mo, and 1y, and parent stool samples were screened for E. coli resistance to tetracycline, ampicillin, co-trimoxazole, and cefazoline by culture, and isolates were further investigated for multiresistance by disc diffusion method. Pulsed-field gel electrophoresis was performed to identify persistent and transmitted strains. Genetic comparison of resistant and transmitted strains was done by multilocus sequence typing (MLST) and strains were further investigated for extra- and intra-intestinal virulence factors by multiplex PCR.

Results

Forty-seven (33%) neonatal meconium samples contained resistant E. coli. Prevalence increased continuously: After 1y, resistance proportion (tetracycline 80%, ampicillin 72%, co-trimoxazole 66%, cefazoline 35%) almost matched those in parents. In 8 infants (6%), identical E. coli strains were found in at least 3 sampling time points (suggesting persistence). Transmission of resistant E. coli from parents to child was observed in only 8 families. MLST showed high diversity. We could not identify any virulence genes or factors associated with persistence, or transmission of resistant E. coli. Full-term, vaginal birth and birth in rural hospital were identified as risk factors for early childhood colonization with resistant E. coli.

Conclusion

One third of healthy Thai neonates harboured antibiotic-resistant E. coli in meconium. The proportion of resistant E. coli increased during the first year of life almost reaching the value in adults. We hypothesize that enhancement of infection control measures and cautious use of antibiotics may help to control further increase of resistance.

SUBMITTER: Hetzer B

PROVIDER: S-EPMC6528363 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Publications

"Enhanced acquisition of antibiotic-resistant intestinal <i>E. coli</i> during the first year of life assessed in a prospective cohort study".

Hetzer Benjamin B Orth-Höller Dorothea D Würzner Reinhard R Kreidl Peter P Lackner Michaela M Müller Thomas T Knabl Ludwig L Geisler-Moroder Daniel Rudolf DR Mellmann Alexander A Sesli Özcan Ö Holzknecht Jeanett J Noce Damia D Boonpala Orawan O Akarathum Noppadon N Chotinaruemol Somporn S Prelog Martina M Oberdorfer Peninnah P

Antimicrobial resistance and infection control 20190520

<h4>Background</h4>Increasing bacterial resistance to antibiotics is a serious problem worldwide. We sought to record the acquisition of antibiotic-resistant <i>Escherichia coli</i> (<i>E. coli</i>) in healthy infants in Northern Thailand and investigated potential determinants.<h4>Methods</h4>Stool samples from 142 infants after birth, at ages 2wk, 2mo, 4 to 6mo, and 1y, and parent stool samples were screened for <i>E. coli</i> resistance to tetracycline, ampicillin, co-trimoxazole, and cefazol ...[more]

PMID: 31139362

Similar Datasets

Project description:Background Antibiotic resistance, an increasing challenge, is not only a national threat but also a global threat. Carriage of resistance genes is not limited to adults alone, various microbiota niches present in the body system of children have been found to harbor bacteria carrying resistant genes, especially, their gut microbiota. This study aims to identify selected antibiotic-resistant genes from the fecal samples of infants and the association of antibiotics use with the occurrence of resistant genes in the infant’s gut. Methods A total number of 172 metagenomic DNA samples previously extracted from stool samples of 28 Nigerian babies longitudinally within their first year of life were screened for the presence of ESBL genes (blaSHV, blaTEM, and blaCTX-M), PMQR genes (qnrA, qnrB, qnrS, qepA), ribosomal protection protein tetracycline resistance gene, (RPP) β-lactamase (blaZ), macrolide (ermA, ermB, mefA/E), aminoglycoside modifying enzymes gentR (aac(6′)/aph(2″)) and dfrA genes by PCR. Nineteen (19) of the 28 babies used antibiotics during the study. The association between antibiotic use by the babies within the first year of life and occurrence of resistant genes were analyzed by Spearman rank correlation. Results One hundred and twenty-two (122) samples (71%) out of the 172 isolates had antibiotic-resistance genes. PMQR genes were absent in all the samples. Three isolates had blaTEM gene, nine isolates had blaSHV gene, six isolates had blaCTX-M gene and 19 isolates had dfrA gene, 31 samples had tet gene, 29 samples had mef gene, 27 samples had ermB gene, four samples had ermA gene, 13 samples had blaZ gene and 16 samples had aac gene. The babies whose samples had resistant genes used antibiotics in the same months the samples were collected. Interestingly, the 11 babies whose samples had the dfrA gene all used antibiotics in the same months their samples were collected but none of them used trimethoprim/sulfamethoxazole antibiotic. The overall correlation matrix of the babies showed a strong association between antibiotic use (AU) and antibiotic use presence of resistance genes (AUPRG) with a coefficient of 0.89. Antibiotic-resistant genes are present in the gut of infants and their occurrence is strongly connected with antibiotic use by infants.

Dataset Information

"Enhanced acquisition of antibiotic-resistant intestinal E. coli during the first year of life assessed in a prospective cohort study".

Background

Methods

Results

Conclusion

Publications

"Enhanced acquisition of antibiotic-resistant intestinal <i>E. coli</i> during the first year of life assessed in a prospective cohort study".

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

"Enhanced acquisition of antibiotic-resistant intestinal E. coli during the first year of life assessed in a prospective cohort study".

Background

Methods

Results

Conclusion

Publications

"Enhanced acquisition of antibiotic-resistant intestinal &lt;i&gt;E. coli&lt;/i&gt; during the first year of life assessed in a prospective cohort study".

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

"Enhanced acquisition of antibiotic-resistant intestinal <i>E. coli</i> during the first year of life assessed in a prospective cohort study".