Dataset Information

Microsatellite Instability Is Associated With the Presence of Lynch Syndrome Pan-Cancer.

ABSTRACT:

Purpose

Microsatellite instability (MSI) and/or mismatch repair deficiency (MMR-D) testing has traditionally been performed in patients with colorectal (CRC) and endometrial cancer (EC) to screen for Lynch syndrome (LS)-associated cancer predisposition. The recent success of immunotherapy in high-frequency MSI (MSI-H) and/or MMR-D tumors now supports testing for MSI in all advanced solid tumors. The extent to which LS accounts for MSI-H across heterogeneous tumor types is unknown. Here, we establish the prevalence of LS across solid tumors according to MSI status.

Methods

MSI status was determined using targeted next-generation sequencing, with tumors classified as MSI-H, MSI-indeterminate, or microsatellite-stable. Matched germline DNA was analyzed for mutations in LS-associated mismatch repair genes ( MLH1, MSH2, MSH6, PMS2, EPCAM). In patients with LS with MSI-H/I tumors, immunohistochemical staining for MMR-D was assessed.

Results

Among 15,045 unique patients (more than 50 cancer types), LS was identified in 16.3% (53 of 326), 1.9% (13 of 699), and 0.3% (37 of 14,020) of patients with MSI-H, MSI-indeterminate, and microsatellite-stable tumors, respectively ( P < .001). Among patients with LS with MSI-H/I tumors, 50% (33 of 66) had tumors other than CRC/EC, including urothelial, prostate, pancreas, adrenocortical, small bowel, sarcoma, mesothelioma, melanoma, gastric, and germ cell tumors. In these patients with non-CRC/EC tumors, 45% (15 of 33) did not meet LS genetic testing criteria on the basis of personal/family history. Immunohistochemical staining of LS-positive MSI-H/I tumors demonstrated MMR-D in 98.2% (56 of 57) of available cases.

Conclusion

MSI-H/MMR-D is predictive of LS across a much broader tumor spectrum than currently appreciated. Given implications for cancer surveillance and prevention measures in affected families, these data support germline genetic assessment for LS for patients with an MSI-H/MMR-D tumor, regardless of cancer type or family cancer history.

SUBMITTER: Latham A

PROVIDER: S-EPMC6553803 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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Publications

Microsatellite Instability Is Associated With the Presence of Lynch Syndrome Pan-Cancer.

Latham Alicia A Srinivasan Preethi P Kemel Yelena Y Shia Jinru J Bandlamudi Chaitanya C Mandelker Diana D Middha Sumit S Hechtman Jaclyn J Zehir Ahmet A Dubard-Gault Marianne M Tran Christina C Stewart Carolyn C Sheehan Margaret M Penson Alexander A DeLair Deborah D Yaeger Rona R Vijai Joseph J Mukherjee Semanti S Galle Jesse J Dickson Mark A MA Janjigian Yelena Y O'Reilly Eileen M EM Segal Neil N Saltz Leonard B LB Reidy-Lagunes Diane D Varghese Anna M AM Bajorin Dean D Carlo Maria I MI Cadoo Karen K Walsh Michael F MF Weiser Martin M Aguilar Julio Garcia JG Klimstra David S DS Diaz Luis A LA Baselga Jose J Zhang Liying L Ladanyi Marc M Hyman David M DM Solit David B DB Robson Mark E ME Taylor Barry S BS Offit Kenneth K Berger Michael F MF Stadler Zsofia K ZK

Journal of clinical oncology : official journal of the American Society of Clinical Oncology 20181030 4

<h4>Purpose</h4>Microsatellite instability (MSI) and/or mismatch repair deficiency (MMR-D) testing has traditionally been performed in patients with colorectal (CRC) and endometrial cancer (EC) to screen for Lynch syndrome (LS)-associated cancer predisposition. The recent success of immunotherapy in high-frequency MSI (MSI-H) and/or MMR-D tumors now supports testing for MSI in all advanced solid tumors. The extent to which LS accounts for MSI-H across heterogeneous tumor types is unknown. Here, ...[more]

PMID: 30376427

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Project description:BackgroundLynch syndrome (LS) is one of the most common hereditary cancer syndromes worldwide. Dominantly inherited mutation in one of four DNA mismatch repair genes combined with somatic events leads to mismatch repair deficiency and microsatellite instability (MSI) in tumours. Due to a high lifetime risk of cancer, regular surveillance plays a key role in cancer prevention; yet the observation of frequent interval cancers points to insufficient cancer prevention by colonoscopy-based methods alone. This study aimed to identify precancerous functional changes in colonic mucosa that could facilitate the monitoring and prevention of cancer development in LS.MethodsThe study material comprised colon biopsy specimens (n = 71) collected during colonoscopy examinations from LS carriers (tumour-free, or diagnosed with adenoma, or diagnosed with carcinoma) and a control group, which included sporadic cases without LS or neoplasia. The majority (80%) of LS carriers had an inherited genetic MLH1 mutation. The remaining 20% included MSH2 mutation carriers (13%) and MSH6 mutation carriers (7%). The transcriptomes were first analysed with RNA-sequencing and followed up with Gorilla Ontology analysis and Reactome Knowledgebase and Ingenuity Pathway Analyses to detect functional changes that might be associated with the initiation of the neoplastic process in LS individuals.FindingsWith pathway and gene ontology analyses combined with measurement of mitotic perimeters from colonic mucosa and tumours, we found an increased tendency to chromosomal instability (CIN), already present in macroscopically normal LS mucosa. Our results suggest that CIN is an earlier aberration than MSI and may be the initial cancer driving aberration, whereas MSI accelerates tumour formation. Furthermore, our results suggest that MLH1 deficiency plays a significant role in the development of CIN.InterpretationThe results validate our previous findings from mice and highlight early mitotic abnormalities as an important contributor and precancerous marker of colorectal tumourigenesis in LS.FundingThis work was supported by grants from the Jane and Aatos Erkko Foundation, the Academy of Finland (330606 and 331284), Cancer Foundation Finland sr, and the Sigrid Jusélius Foundation. Open access is funded by Helsinki University Library.

Dataset Information

Microsatellite Instability Is Associated With the Presence of Lynch Syndrome Pan-Cancer.

Purpose

Methods

Results

Conclusion

Publications

Microsatellite Instability Is Associated With the Presence of Lynch Syndrome Pan-Cancer.

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