A new mouse model to study the role of ectopic Nanos3 expression in cancer.

ABSTRACT:

Background

NANOS3 is a gene conserved throughout evolution. Despite the quite low conservation of Nanos sequences between different organisms and even between Nanos paralogs, their role in germ cell development is remarkably universal. Human Nanos3 expression is normally restricted to the gonads and the brain. However, ectopic activation of this gene has been detected in various human cancers. Until now, Nanos3 and other Nanos proteins have been studied almost exclusively in germ cell development.

Methods

Transgenic mice were generated by targeted insertion of a human Nanos3 cDNA into the ROSA26 locus. The transgene could be spatiotemporally induced by Cre recombinase activity removing an upstream floxed STOP cassette. A lung tumor model with ectopic Nanos3 expression was ba

SUBMITTER: Andries V 

PROVIDER: S-EPMC6580527 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature