Genomic and transcriptional heterogeneity of multifocal hepatocellular carcinoma.

ABSTRACT:

Background

Hepatocellular carcinoma (HCC) often presents with multiple nodules within the liver, with limited effective interventions. The high genetic heterogeneity of HCC might be the major cause of treatment failure. We aimed to characterize genomic heterogeneity, infer clonal evolution, investigate RNA expression pattern and explore tumour immune microenvironment profile of multifocal HCC.

Patients and methods

Whole-exome sequencing and RNA sequencing were carried out in 34 tumours and 6 adjacent normal liver tissue samples from 6 multifocal HCC patients. Protein expression of Ki67, AFP, P53, Survivin and CD8 was detected by immunohistochemistry. Fluorescence in situ hybridization was carried out to validate the amplification status of sorafenib-targeted genes.

Result

SUBMITTER: Xu LX 

PROVIDER: S-EPMC6594462 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature