Kinetics of Xist-induced gene silencing can be predicted from combinations of epigenetic and genomic features.

ABSTRACT: To initiate X-Chromosome inactivation (XCI), the long noncoding RNA Xist mediates chromosome-wide gene silencing of one X Chromosome in female mammals to equalize gene dosage between the sexes. The efficiency of gene silencing is highly variable across genes, with some genes even escaping XCI in somatic cells. A gene's susceptibility to Xist-mediated silencing appears to be determined by a complex interplay of epigenetic and genomic features; however, the underlying rules remain poorly understood. We have quantified chromosome-wide gene silencing kinetics at the level of the nascent transcriptome using allele-specific Precision nuclear Run-On sequencing (PRO-seq). We have developed a Random Forest machine-learning model that can predict the measured silencing dynamics based o

SUBMITTER: Barros de Andrade E Sousa L 

PROVIDER: S-EPMC6633258 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature