External quality assessment for PML-RARα detection in acute promyelocytic leukemia: Findings and summary.

ABSTRACT:

Background

The confirmation of clinical diagnosis, molecular remission, and sequential minimal residual disease monitoring required PML-RARα detection in acute promyelocytic leukemia (APL). The current status of PML-RARα detection in various laboratories remains unknown.

Methods

In 2018, external quality assessment (EQA) for PML-RARα detection was carried out in China. Three EQA sample panels for PML-RARα isoform L/S/V were prepared by different mock leukocyte samples. The performances of PML-RARα detection, including admission screening, and qualitative and quantitative detection by real-time quantitative reverse transcription PCR (RT-qPCR), were assessed based on APL simulated clinical case.

Results

The mock leukocyte samples met the requirements of a clinically qua

SUBMITTER: Wu Q 

PROVIDER: S-EPMC6642306 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature