Alternol eliminates excessive ATP production by disturbing Krebs cycle in prostate cancer.

ABSTRACT:

Background

Alternol is a natural compound isolated from fermentation products of a mutant fungus. Our previous studies demonstrated that Alternol specifically kills cancer cells but spares benign cells.

Methods

To investigate the mechanism underlying alternol-induced cancer cell-specific killing effect, we took a comprehensive strategy to identify Alternol's protein targets in prostate cancer cells, including PC-3, C4-2, and 22RV1, plus benign BPH1 cell lines. Major experimental techniques included biotin-streptavidin pulldown assay coupled with mass-spectrometry, in vitro enzyme activity assay for Krebs cycle enzymes and gas chromatography-mass spectrometry (GC-MS) for metabolomic analysis.

Results

Among 14 verified protein targets, four were Krebs cycle enzymes, fum

SUBMITTER: Li C 

PROVIDER: S-EPMC6644699 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature