Reduction-Responsive DNA Duplex Containing O ⁶-Nitrobenzyl-Guanine.

ABSTRACT: Stimuli-controlled structural transitions of nucleic acids have received growing attentions owing to their potential applications in the fields of chemical and synthetic biology. Here, we describe the development of reduction-responsive deoxyribonucleic acid (DNA) duplexes, in which guanine rings bearing a reduction-responsive cleavable nitrobenzyl (NB) group at the O ⁶ position (G^NB) are introduced at defined positions. We demonstrate that the artificial NB group can be removed in response to reduction stimulus without the dissociation of the intermolecular duplex structure, which comprises a G-quadruplex forming nucleic acid strand with one G^NB and its complementary sequence with one mismatch pair. Meanwhile, another duplex that comprised a G-quadr

SUBMITTER: Hayakawa Y 

PROVIDER: S-EPMC6645092 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature