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Generation of human induced pluripotent stem cells from individuals with a homozygous CCR5Δ32 mutation.

ABSTRACT: Chemokine receptor 5 (CCR5) is the primary coreceptor for HIV entry into macrophages. Individuals with a homozygous deletion of 32 bp in the CCR5 gene (CCR5Δ32) are highly resistant to HIV infection (Samson et al., 1996). Allogeneic stem cell transplantation from a healthy donor with the homozygous CCR5Δ32 variant to an HIV positive individual has demonstrated efficient long-term control of HIV. We identified three individuals with this homozygous CCR5Δ32 variant, and successfully generated induced pluripotent stem cell (iPSC) lines from their dermal fibroblasts. The iPSCs lines carrying homozygous CCR5Δ32 variant displayed phenotypically normal and the potential to differentiation toward the three germ layers.

SUBMITTER: Chen G

PROVIDER: S-EPMC6662727 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Generation of human induced pluripotent stem cells from individuals with a homozygous CCR5Δ32 mutation.

Chen Guibin G Jin Hui H Yu Zhen Z Liu Yangtengyu Y Li Zhongwen Z Navarengom Keron K Schwartzbeck Robin R Dmitrieva Natalia N Cudrici Cornelia C Ferrante Elisa A EA Biesecker Leslie G LG Yang Dan D Boehm Manfred M

Stem cell research 20190605

Chemokine receptor 5 (CCR5) is the primary coreceptor for HIV entry into macrophages. Individuals with a homozygous deletion of 32 bp in the CCR5 gene (CCR5Δ32) are highly resistant to HIV infection (Samson et al., 1996). Allogeneic stem cell transplantation from a healthy donor with the homozygous CCR5Δ32 variant to an HIV positive individual has demonstrated efficient long-term control of HIV. We identified three individuals with this homozygous CCR5Δ32 variant, and successfully generated indu ...[more]

PMID: 31234109

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