Novel biotechnological glucosylation of high-impact aroma chemicals, 3(2H)- and 2(5H)-furanones.

ABSTRACT: Glucosyltransferases are versatile biocatalysts to chemically modify small molecules and thus enhance their water solubility and structural stability. Although the genomes of all organisms harbor a multitude of glucosyltransferase genes, their functional characterization is hampered by the lack of high-throughput in-vivo systems to rapidly test the versatility of the encoded proteins. We have developed and applied a high-throughput whole cell biotransformation system to screen a plant glucosyltransferase library. As proof of principle, we identified 25, 24, 15, and 18 biocatalysts transferring D-glucose to sotolone, maple furanone, furaneol and homofuraneol, four highly appreciated flavor compounds, respectively. Although these 3(2H)- and 2(5H)-furanones have extremely low odor thresholds

SUBMITTER: Effenberger I 

PROVIDER: S-EPMC6662797 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature