High temperature suppressed SSC self-renewal through S phase cell cycle arrest but not apoptosis.

ABSTRACT:

Background

High temperature has a very adverse effect on mammalian spermatogenesis and eventually leads to sub- or infertility through either apoptosis or DNA damage. However, the direct effects of heat stress on the development of spermatogonial stem cells (SSCs) are still unknown because SSCs are rare in the testes.

Methods

In the present study, we first used in vitro-cultured SSCs to study the effect of heat shock treatment on SSC development. Then, we used RNA-Seq analysis to identify new genes or signalling pathways implicated in the heat stress response.

Results

We found that 45 min of 43 °C heat shock treatment significantly inhibited the proliferation of SSCs 2 h after treatment but did not lead to apoptosis. In total, 17,822 genes were identified by RNA-Seq a

SUBMITTER: Wang J 

PROVIDER: S-EPMC6664773 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature