The thioredoxin (Trx) redox state sensor protein can visualize Trx activities in the light/dark response in chloroplasts.

ABSTRACT: Thiol-based redox regulation via ferredoxin-thioredoxin (Trx) reductase/Trx controls various functions in chloroplasts in response to light/dark changes. Trx is a key factor of this regulatory system, and five Trx subtypes, including 10 isoforms, have been identified as chloroplast-localized forms in Arabidopsis thaliana These subtypes display distinct target selectivity, and, consequently, they form a complicated redox regulation network in chloroplasts. In this study, we developed a FRET-based sensor protein by combining CFP, YFP, and the N-terminal region of CP12, a redox-sensitive regulatory and Trx-targeted protein in chloroplasts. This sensor protein enabled us to monitor the redox change of chloroplast thioredoxin in vivo, and we therefore designated this protein "chan

SUBMITTER: Sugiura K 

PROVIDER: S-EPMC6690705 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature