Endothelial TRPV4 channels modulate vascular tone by Ca²⁺ -induced Ca²⁺ release at inositol 1,4,5-trisphosphate receptors.

ABSTRACT:

Background and purpose

The TRPV4 ion channels are Ca²⁺ permeable, non-selective cation channels that mediate large, but highly localized, Ca²⁺ signals in the endothelium. The mechanisms that permit highly localized Ca²⁺ changes to evoke cell-wide activity are incompletely understood. Here, we tested the hypothesis that TRPV4-mediated Ca²⁺ influx activates Ca²⁺ release from internal Ca²⁺ stores to generate widespread effects.

Experimental approach

Ca²⁺ signals in large numbers (~100) of endothelial cells in intact arteries were imaged and analysed separately.

Key results

Responses to the TRPV4 channel agonist GSK1016790A were heterogeneous across the endothelium. In activated cells, Ca^2+<

SUBMITTER: Heathcote HR 

PROVIDER: S-EPMC6692577 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature