Transregulation of microRNA miR-21 promoter by AP-1 transcription factor in cervical cancer cells.

ABSTRACT:

Background

Gene expression profiles have demonstrated that miR-21 expression is altered in almost all types of cancers and it has been classified as an oncogenic microRNA. Persistent HPV infection is the main etiologic agent in cervical cancer and induces genetic instability, including disruption of microRNA gene expression. In the present study, we analyzed the underlying mechanism of how AP-1 transcription factor can active miR-21 gene expression in cervical cancer cells.

Methods

To identify that c-Fos and c-Jun regulate the expression of miR-21 we performed RT-qPCR and western blot assays. We analyzed the interaction of AP-1 with miR-21 promoter by EMSA and ChIP assays and determined the mechanism of its regulation by reporter construct plasmids. We identified the nuclear

SUBMITTER: Del Mar Diaz-Gonzalez S 

PROVIDER: S-EPMC6694678 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature