Ontology highlight
ABSTRACT:
SUBMITTER: Quan J
PROVIDER: S-EPMC6698650 | biostudies-literature | 2019 Aug
REPOSITORIES: biostudies-literature
Nucleic acids research 20190801 14
The growing prevalence of deadly microbes with resistance to previously life-saving drug therapies is a dire threat to human health. Detection of low abundance pathogen sequences remains a challenge for metagenomic Next Generation Sequencing (NGS). We introduce FLASH (Finding Low Abundance Sequences by Hybridization), a next-generation CRISPR/Cas9 diagnostic method that takes advantage of the efficiency, specificity and flexibility of Cas9 to enrich for a programmed set of sequences. FLASH-NGS a ...[more]