Construction of industrial Saccharomyces cerevisiae strains for the efficient consolidated bioprocessing of raw starch.

ABSTRACT:

Background

Consolidated bioprocessing (CBP) combines enzyme production, saccharification and fermentation into a one-step process. This strategy represents a promising alternative for economic ethanol production from starchy biomass with the use of amylolytic industrial yeast strains.

Results

Recombinant Saccharomyces cerevisiae Y294 laboratory strains simultaneously expressing an α-amylase and glucoamylase gene were screened to identify the best enzyme combination for raw starch hydrolysis. The codon optimised Talaromyces emersonii glucoamylase encoding gene (temG_Opt) and the native T. emersonii α-amylase encoding gene (temA) were selected for expression in two industrial S. cerevisiae yeast strains, namely Ethanol Red™ (hereafter

SUBMITTER: Cripwell RA 

PROVIDER: S-EPMC6701143 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature