Mapping histone modifications in low cell number and single cells using antibody-guided chromatin tagmentation (ACT-seq).

ABSTRACT: Modern next-generation sequencing-based methods have empowered researchers to assay the epigenetic states of individual cells. Existing techniques for profiling epigenetic marks in single cells often require the use and optimization of time-intensive procedures such as drop fluidics, chromatin fragmentation, and end repair. Here we describe ACT-seq, a streamlined method for mapping genome-wide distributions of histone tail modifications, histone variants, and chromatin-binding proteins in a small number of or single cells. ACT-seq utilizes a fusion of Tn5 transposase to Protein A that is targeted to chromatin by a specific antibody, allowing chromatin fragmentation and sequence tag insertion specifically at genomic sites presenting the relevant antigen. The Tn5 transposase enables the use

SUBMITTER: Carter B 

PROVIDER: S-EPMC6702168 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature