Physiological Biomimetic Culture System for Pig and Human Heart Slices.

ABSTRACT:

Rationale

Preclinical testing of cardiotoxicity and efficacy of novel heart failure therapies faces a major limitation: the lack of an in situ culture system that emulates the complexity of human heart tissue and maintains viability and functionality for a prolonged time.

Objective

To develop a reliable, easily reproducible, medium-throughput method to culture pig and human heart slices under physiological conditions for a prolonged period of time.

Methods and results

Here, we describe a novel, medium-throughput biomimetic culture system that maintains viability and functionality of human and pig heart slices (300 µm thickness) for 6 days in culture. We optimized the medium and culture conditions with continuous electrical stimulation at 1.2 Hz and oxygenation of the

SUBMITTER: Ou Q 

PROVIDER: S-EPMC6715512 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature