A rotating operant chamber for use with microdialysis.

ABSTRACT:

Background

Recently, the time resolution of microdialysis followed by a chemical separation for quantitative analysis has improved. The advent of faster microdialysis measurements promises to aid in behavioral research on awake animals. However, microdialysis with awake animals generally employs a fluidic commutator (swivel). The swivel's volume is inimical to the time resolution of the measurements.

New method

Animals can be housed in rotating cages so that the swivel is not required, but rotating operant chambers are not available. Here we describe the design and construction of a rotating operant chamber with microdialysis capability. We modified a rotating cage by adding operant behavior testing components to the side of the bowl-shaped cage. A modular on-board controlle

SUBMITTER: Degreef B 

PROVIDER: S-EPMC6717681 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature