Patient-Derived Cancer Organoid Cultures to Predict Sensitivity to Chemotherapy and Radiation.

ABSTRACT:

Purpose

Cancer treatment is limited by inaccurate predictors of patient-specific therapeutic response. Therefore, some patients are exposed to unnecessary side effects and delays in starting effective therapy. A clinical tool that predicts treatment sensitivity for individual patients is needed.

Experimental design

Patient-derived cancer organoids were derived across multiple histologies. The histologic characteristics, mutation profile, clonal structure, and response to chemotherapy and radiation were assessed using bright-field and optical metabolic imaging on spheroid and single-cell levels, respectively.

Results

We demonstrate that patient-derived cancer organoids represent the cancers from which they were derived, including key histologic and molecular features.

SUBMITTER: Pasch CA 

PROVIDER: S-EPMC6726566 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature