Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time.
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ABSTRACT:
SUBMITTER: Bergdahl GE
PROVIDER: S-EPMC6754820 | biostudies-literature | 2019 Oct
REPOSITORIES: biostudies-literature
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Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time.
Bergdahl Gizem Ertürk GE Hedström Martin M Mattiasson Bo B
Applied biochemistry and biotechnology 20190424 2
A capacitive sensor was developed to analyze the presence and enzymatic activity of a model protease from standard solutions by following the degradation of the substrate in real time. The enzyme was chosen based on its specific digestion of the hinge region of immunoglobulin G (IgG). Real-time enzyme activity was monitored by measuring the change in capacitance (∆C) based on the release of IgG fragments after enzymatic digestion by the enzyme. The results indicated that the developed capacitive ...[more]
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