Dataset Information

IL-17 Inversely Correlated with IL-10 via the STAT3 Gene in Pneumocystis-Infected Mice.

ABSTRACT:

Background

Pneumocystis pneumonia (PCP) remains a common opportunistic infection in immunosuppressed individuals. Current studies showed that multiple immune cells and cytokines took part in the host defense against Pneumocystis (PC). However, the roles of IL-17 and IL-10 in the development of PCP have not been elucidated.

Methods

IL-10 and IL-17 levels in serum from PCP mice were detected via ELISA. The percentages of B10 cells, IL-10⁺ macrophages, and IL-10⁺ T cells in the lung from IL-17^-/- PCP mice and Th17 cells and IL-17⁺ γδT cells in IL-10^-/- PCP mice were examined via flow cytometry. Also, antibody neutralization examination was also performed to elucidate the relationship of IL-17 and IL-10 in the PCP model.

Results

We noted the increase of IL-17 and IL-10 levels in serum from mice infected with Pneumocystis. Furthermore, deficiency of IL-17 or IL-10 could lead to the delayed clearance of Pneumocystis and more severed lung damage. Our data also demonstrated that IL-17 deficiency enhanced the serum IL-10 level and the percentages of B10 cells, IL-10⁺ macrophages, and IL-10⁺ T cells in the lung from PCP mice. Interestingly, we also noted an increase of the IL-17 level in serum and Th17 cell and IL-17⁺ γδT cell percentages in the lung from IL-10^-/- PCP mice. Using antibody neutralization experiments, we found that the STAT3 gene might play a critical role in the interplay of IL-17 and IL-10 in PCP.

Conclusion

Taken together, our results demonstrated that IL-17 and IL-10 could play the protective roles in the progression of PCP and the inverse correlation of them might be mediated by STAT3.

SUBMITTER: Rong HM

PROVIDER: S-EPMC6754930 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Publications

IL-17 Inversely Correlated with IL-10 via the STAT3 Gene in <i>Pneumocystis</i>-Infected Mice.

Rong Heng-Mo HM Qian Xiao-Jun XJ Zhang Chao C Li Ting T Tong Zhao-Hui ZH

Mediators of inflammation 20190910

<h4>Background</h4><i>Pneumocystis</i> pneumonia (PCP) remains a common opportunistic infection in immunosuppressed individuals. Current studies showed that multiple immune cells and cytokines took part in the host defense against <i>Pneumocystis</i> (PC). However, the roles of IL-17 and IL-10 in the development of PCP have not been elucidated.<h4>Methods</h4>IL-10 and IL-17 levels in serum from PCP mice were detected via ELISA. The percentages of B10 cells, IL-10<sup>+</sup> macrophages, and IL ...[more]

PMID: 31582901

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