Next-Generation Sequencing for the Diagnosis of Challenging Culture-Negative Endocarditis.

ABSTRACT: Diagnosis of culture-negative infective endocarditis usually implies indirect pathogen identification by serologic or molecular techniques. Clinical metagenomics, relying on next-generation sequencing (NGS) is an emerging approach that allows pathogen identification in challenging situations, as evidenced by a clinical case. We sequenced the DNA extracted from the surgically-removed frozen valve tissue from a patient with suspected infective endocarditis with negative blood and valve cultures. Mapping of the sequence reads against reference genomic sequences, a 16S rRNA gene database and clade-specific marker genes suggested an infection caused by Cardiobacterium hominis.

SUBMITTER: Kolb M 

PROVIDER: S-EPMC6763761 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature