A novel conserved enhancer at zebrafish zic3 and zic6 loci drives neural expression.

ABSTRACT:

Background

Identifying enhancers and deciphering their putative roles represent a major step to better understand the mechanism of metazoan gene regulation, development, and the role of regulatory elements in disease. Comparative genomics and transgenic assays have been used with some success to identify critical regions that are involved in regulating the spatiotemporal expression of genes during embryogenesis.

Results

We identified two novel tetrapod-teleost conserved noncoding elements within the vicinity of the zic3 and zic6 loci in the zebrafish genome and demonstrated their ability to drive tissue-specific expression in a transgenic zebrafish assay. The syntenic analysis and robust green fluorescent expression in the developing habenula in the stable transgenic line we

SUBMITTER: Minhas R 

PROVIDER: S-EPMC6771876 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature