Oxidative Stress in Response to Saturated Fat Ingestion Is Linked to Insulin Resistance and Hyperandrogenism in Polycystic Ovary Syndrome.

ABSTRACT:

Context

Oxidative stress and insulin resistance are often present in polycystic ovary syndrome (PCOS).

Objective

We determined the effect of saturated fat ingestion on leukocytic reactive oxygen species (ROS) generation, p47phox expression, and circulating thiobarbituric acid-reactive substances (TBARS) in women with PCOS.

Design

Cross-sectional study.

Setting

Academic medical center.

Patients

Twenty women of reproductive age with PCOS (10 lean, 10 with obesity) and 19 ovulatory control subjects (10 lean, 9 with obesity).

Main outcome measures

ROS generation and p47phox mRNA and protein content were quantified in leukocytes, and TBARS was measured in plasma from blood drawn while the subjects were fasting and 2, 3, and 5 hours after saturated fat

SUBMITTER: Gonzalez F 

PROVIDER: S-EPMC6773460 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature