Comparison of cytosine base editors and development of the BEable-GPS database for targeting pathogenic SNVs.

ABSTRACT: A variety of base editors have been developed to achieve C-to-T editing in different genomic contexts. Here, we compare a panel of five base editors on their C-to-T editing efficiencies and product purity at commonly editable sites, including some human pathogenic C-to-T mutations. We further profile the accessibilities of 20 base editors to all possible pathogenic mutations in silico. Finally, we build the BEable-GPS (Base Editable prediction of Global Pathogenic SNVs) database for users to select proper base editors to model or correct disease-related mutations. The in vivo comparison and in silico profiling catalog the availability of base editors and their broad applications in biomedical studies.

SUBMITTER: Wang Y 

PROVIDER: S-EPMC6806563 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature