High-Resolution Melting Analysis as a Developed Method for Genotyping the PD Susceptibility Loci in LRRK2 Gene.

ABSTRACT:

Background

Single-nucleotide polymorphisms (SNPs) have been reported as a highly relevant point for the mechanisms of Parkinson's disease (PD). The invention of saturating dye makes it possible to identify heteroduplex DNA without redistribution during melting, which allows using high-resolution melting (HRM) to detect SNPs. However, the HRM analysis for detection of those SNPs associated with PD was rarely applied.

Methods

Two SNPs, G2385R and R1628P, located in leucine-rich repeat kinase 2 (LRRK2) gene were individually and multiplexedly genotyped using HRM analysis. The sequence variant observed in unexpected HRM curves was confirmed by DNA sequencing.

Results

HRM analysis identified successfully all genotypes both on R1628P and G2385R loci. The unexpected HRM curv

SUBMITTER: Jiang E 

PROVIDER: S-EPMC6807008 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature