Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria.

ABSTRACT: The ability to determine full-length nucleotide composition of individual RNA molecules is essential for understanding the architecture and function of a transcriptome. However, experimental approaches capable of capturing the sequences of both 5' and 3' termini of the same transcript remain scarce. In the present study, simultaneous 5' and 3' end sequencing (SEnd-seq)-a high-throughput and unbiased method that simultaneously maps transcription start and termination sites with single-nucleotide resolution-is presented. Using this method, a comprehensive view of the Escherichia coli transcriptome was obtained, which displays an unexpected level of complexity. SEnd-seq notably expands the catalogue of transcription start sites and termination sites, defines unique transcription units and det

SUBMITTER: Ju X 

PROVIDER: S-EPMC6814526 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature