Rapid detection of the New Delhi metallo-b-lactamase 1 (NDM-1) gene by loop-mediated isothermal amplification (LAMP).

ABSTRACT:

Background

New Delhi Metallo-b-lactamase (NDM-1) is an enzyme emerging around the world conferring resistance to a wide range of β-lactams agents and whose early detection is extremely important. We proposed to standardize the detection of the bla_NDM-1 gene using the LOOP-mediated isothermal amplification technique (LAMP).

Methods

In all, 14 Gram-negative bacterial strains isolated from patients presenting pneumonia associated with mechanical ventilation were used for the bla_NDM-1 standardization by LAMP. Klebsiella pneumoniae ATCC BAA-2473 and two clinical strains were used as a positive control. All results were compared to the reaction in polymerase chain reaction (PCR), considered gold standard for this detection.

Results

SUBMITTER: Moreira MG 

PROVIDER: S-EPMC6816916 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature