Genomic integrity of human induced pluripotent stem cells: Reprogramming, differentiation and applications.

ABSTRACT: Ten years after the initial generation of induced pluripotent stem cells (hiPSCs) from human tissues, their potential is no longer questioned, with over 15000 publications listed on PubMed, covering various fields of research; including disease modeling, cell therapy strategies, pharmacology/toxicology screening and 3D organoid systems. However, despite evidences that the presence of mutations in hiPSCs should be a concern, publications addressing genomic integrity of these cells represent less than 1% of the literature. After a first overview of the mutation types currently reported in hiPSCs, including karyotype abnormalities, copy number variations, single point mutation as well as uniparental disomy, this review will discuss the impact of reprogramming parameters such as starting cell

SUBMITTER: Steichen C 

PROVIDER: S-EPMC6828592 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature