Raman-activated cell sorting and metagenomic sequencing revealing carbon-fixing bacteria in the ocean.

ABSTRACT: It is of great significance to understand CO₂ fixation in the oceans. Using single cell Raman spectra (SCRS) as biochemical profiles, Raman activated cell ejection (RACE) was able to link phenotypes and genotypes of cells. Here, we show that mini-metagenomic sequences from RACE can be used as a reference to reconstruct nearly complete genomes of key functional bacteria by binning shotgun metagenomic sequencing data. By applying this approach to ¹³ C bicarbonate spiked seawater from euphotic zone of the Yellow Sea of China, the dominant bacteria Synechococcus spp. and Pelagibacter spp. were revealed and both of them contain carotenoid and were able to incorporate ¹³ C into the cells at the same time. Genetic analysis of the reconstructed genomes suggests tha

SUBMITTER: Jing X 

PROVIDER: S-EPMC6849569 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature