Sequencing of animal viruses: quality data assurance for NGS bioinformatics.

ABSTRACT:

Background

Next generation sequencing (NGS) is becoming widely used among diagnostics and research laboratories, and nowadays it is applied to a variety of disciplines, including veterinary virology. The NGS workflow comprises several steps, namely sample processing, library preparation, sequencing and primary/secondary/tertiary bioinformatics (BI) analyses. The latter is constituted by a complex process extremely difficult to standardize, due to the variety of tools and metrics available. Thus, it is of the utmost importance to assess the comparability of results obtained through different methods and in different laboratories. To achieve this goal, we have organized a proficiency test focused on the bioinformatics components for the generation of complete genome sequences of salm

SUBMITTER: Zamperin G 

PROVIDER: S-EPMC6868765 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature