Long-term impact of tylosin on fecal microbiota and fecal bile acids of healthy dogs.

ABSTRACT: BACKGROUND:Tylosin is commonly prescribed to dogs with diarrhea. Orally administered antibiotics may alter the intestinal microbiota, which is responsible for crucial key bile acid (BA) biotransformation reactions. OBJECTIVES:To prospectively evaluate the impact of tylosin administration on fecal microbiota and unconjugated bile acids (UBAs) over time. ANIMALS:Sixteen healthy adult dogs. METHODS:Prospective, randomized controlled clinical trial. Dogs were randomized to receive 20?mg/kg of tylosin or a placebo capsule PO q12h for 7?days while undergoing daily fecal scoring. Fecal samples were collected on days 0, 7, 21, and 63. The microbiota was assessed using quantitative PCR and 16S rRNA gene sequencing. Unconjugated BAs were assessed using gas chromatography-mass spectrometry (GC-MS). RESULTS:Fecal scores were unchanged during placebo and tylosin administration. In the placebo group, no significant changes were observed in fecal microbiota or UBA concentrations. Day 7 samples from tylosin-exposed dogs exhibited decreased bacterial diversity (observed species, Chao1, Shannon, P?

SUBMITTER: Manchester AC 

PROVIDER: S-EPMC6872867 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature