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Quantitative Ultrasound Detects Smooth Muscle Activity at the Cervical Internal Os in Vitro.

ABSTRACT: The cervix has two biomechanical functions: to remain closed while the fetus develops throughout pregnancy, and to open for delivery of the fetus at full term. This dual function is principally attributed to collagen within the extracellular matrix (ECM). However, recent evidence suggests that other ECM, and non-ECM, components play a role as well. One component is smooth muscle cells arranged circumferentially near the internal os. In this study, we investigate correlations between cervical smooth muscle cell force generation and the effective scatterer diameter (ESD), a quantitative ultrasound parameter directly related to the acoustic impedance distribution and, therefore, a potential biomarker of muscle contractility. Using whole cervical slices (N = 5), we determined significant positive correlations (quantified with Pearson's r) between muscle force generation and ESD immediately after administration of oxytocin (median r = 0.90). In summary, the ESD may prove a useful biomarker for studying structure and function of cervical smooth muscle in vivo.

SUBMITTER: Santoso AP

PROVIDER: S-EPMC6879854 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Quantitative Ultrasound Detects Smooth Muscle Activity at the Cervical Internal Os in Vitro.

Santoso Andrew P AP Vink Joy Y JY Gallos George G Feltovich Helen H Hall Timothy J TJ

Ultrasound in medicine & biology 20191024 1

The cervix has two biomechanical functions: to remain closed while the fetus develops throughout pregnancy, and to open for delivery of the fetus at full term. This dual function is principally attributed to collagen within the extracellular matrix (ECM). However, recent evidence suggests that other ECM, and non-ECM, components play a role as well. One component is smooth muscle cells arranged circumferentially near the internal os. In this study, we investigate correlations between cervical smo ...[more]

PMID: 31668428

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Project description:BackgroundCurrently, the activity of juvenile dermatomyositis (JDM) is mainly assessed based on clinical manifestations, creatine kinase (CK) level, and magnetic resonance imaging (MRI), but certain limitations arise in these approaches for children. Thus, this cross-sectional study aimed to explore the value of ultrasound in evaluating muscle inflammation via the dynamic analysis of muscle ultrasound characteristics in children with active or stable JDM.MethodsThe data of a group of children who were diagnosed with JDM and admitted to the Rheumatology and Immunology Department of the Capital Pediatric Research Institute Children's Hospital between June 2022 and November 2023, and a normal control group were collected. The clinical, ultrasound, and laboratory data of the children with active and stable JDM were collected and compared with those of a normal control group. Muscle thickness (MT), fascia thickness (FT), muscle echo intensity (EI), muscle microvascular flow imaging (MVFI) distribution, and the blood flow resistance index (RI) were measured via ultrasound for the comparative analysis. A Spearman correlation analysis was conducted to assess the correlation between the ultrasound parameters in the JDM patients, and muscle scores and laboratory indicators of disease activity. Receiver operating characteristic (ROC) curves were generated for the ultrasound parameters. Five active JDM children were dynamically followed up.ResultsThe data of 26 children with active JDM, 29 with stable JDM, and 31 normal control children were collected. The patients with active JDM had significantly greater EI (median 68.9 vs. 47.4, P<0.01), increased FT (median 0.25 vs. 0.15, P<0.01), and an increased MVFI distribution (P<0.01). The Spearman correlation analysis revealed a negative correlation between increased EI, FT, and MVFI distribution, and the Childhood Myositis Assessment Scale (CMAS) score (R=-0.662, -0.673, -0.667, all P<0.05). There was a statistically significant difference in EI between the stable JDM children and healthy children (median 47.4 vs. 39.0, P<0.05). During the follow-up period, two children with slow fasciitis resolution developed soft tissue calcification.ConclusionsEI, FT, and MVFI distribution can be used to assess the activity status of individuals with JDM, and are correlated with clinical activity indices. EI may be abnormal even in children with a stable clinical condition, which suggests that ultrasound may more accurately reflect muscle status. Children with long-term unresolved fasciitis may be at risk of developing calcification.

Project description:BackgroundLung ultrasound (LUS) may not detect small, dynamic changes in lung volume. Mean greyscale measurement using computer-assisted image analysis (Q-LUSMGV) may improve the precision of these measurements.MethodsPreterm lambs (n = 40) underwent LUS of the dependent or non-dependent lung during static pressure-volume curve mapping. Total and regional lung volumes were determined using the super-syringe technique and electrical impedance tomography. Q-LUSMGV and gold standard measurements of lung volume were compared in 520 images.ResultsDependent Q-LUSMGV moderately correlated with total lung volume (rho = 0.60, 95% CI 0.51-0.67) and fairly with right whole (rho = 0.39, 0.27-0.49), central (rho = 0.38, 0.27-0.48), ventral (rho = 0.41, 0.31-0.51) and dorsal regional lung volumes (rho = 0.32, 0.21-0.43). Non-dependent Q-LUSMGV moderately correlated with total lung volume (rho = 0.57, 0.48-0.65) and fairly with right whole (rho = 0.43, 0.32-0.52), central (rho = 0.46, 0.35-0.55), ventral (rho = 0.36, 0.25-0.47) and dorsal lung volumes (rho = 0.36, 0.25-0.47). All correlation coefficients were statistically significant. Distinct inflation and deflation limbs, and sonographic pulmonary hysteresis occurred in 95% of lambs. The greatest changes in Q-LUSMGV occurred at the opening and closing pressures.ConclusionQ-LUSMGV detected changes in total and regional lung volume and offers objective quantification of LUS images, and may improve bedside discrimination of real-time changes in lung volume.ImpactLung ultrasound (LUS) offers continuous, radiation-free imaging that may play a role in assessing lung recruitment but may not detect small changes in lung volume. Mean greyscale image analysis using computer-assisted quantitative LUS (Q-LUSMGV) moderately correlated with changes in total and regional lung volume. Q-LUSMGV identified opening and closing pressure and pulmonary hysteresis in 95% of lambs. Computer-assisted image analysis may enhance LUS estimation of lung recruitment at the bedside. Future research should focus on improving precision prior to clinical translation.

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Quantitative Ultrasound Detects Smooth Muscle Activity at the Cervical Internal Os in Vitro.

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Quantitative Ultrasound Detects Smooth Muscle Activity at the Cervical Internal Os in Vitro.

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