Reprogramming of Cancer Cells into Induced Pluripotent Stem Cells Questioned.

ABSTRACT:

Background and objectives

Several recent studies have claimed that cancer cells can be reprogrammed into induced pluripotent stem cells (iPSCs). However, in most cases, cancer cells seem to be resistant to cellular reprogramming. Furthermore, the underlying mechanisms of limited reprogramming in cancer cells are largely unknown. Here, we identified the candidate barrier genes and their target genes at the early stage of reprogramming for investigating cancer reprogramming.

Methods

We tried induction of pluripotency in normal human fibroblasts (BJ) and both human benign (MCF10A) and malignant (MCF7) breast cancer cell lines using a classical retroviral reprogramming method. We conducted RNA-sequencing analysis to compare the transcriptome of the three cell lines at early stag

SUBMITTER: Bang JS 

PROVIDER: S-EPMC6881048 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature