One-Pot SELEX: Identification of Specific Aptamers against Diverse Steroid Targets in One Selection.

ABSTRACT: Aptamers are well-established biorecognition molecules used in a wide variety of applications for the detection of their respective targets. However, individual SELEX processes typically performed for the identification of aptamers for each target can be quite time-consuming, labor-intensive, and costly. An alternative strategy is proposed herein for the simultaneous identification of different aptamers binding distinct but structurally similar targets in one single selection. This one-pot SELEX approach, using the steroids estradiol, progesterone, and testosterone as model targets, was achieved by combining the benefits of counter-SELEX with the power of next-generation sequencing and bioinformatics analysis. The pools from the last stage of the selection were compared in order to discove

SUBMITTER: Jauset-Rubio M 

PROVIDER: S-EPMC6893951 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature