Skeletal muscle unloading results in increased mitophagy and decreased mitochondrial biogenesis regulation.

ABSTRACT:

Introduction

Physical inactivity significantly contributes to loss of muscle mass and performance in bed-bound patients. Loss of skeletal muscle mitochondrial content has been well-established in muscle unloading models, but the underlying molecular mechanism remains unclear. We hypothesized that apparent unloading-induced loss of muscle mitochondrial content is preceded by increased mitophagy- and decreased mitochondrial biogenesis-signaling during the early stages of unloading.

Methods

We analyzed a comprehensive set of molecular markers involved in mitochondrial-autophagy, -biogenesis, -dynamics, and -content, in the gastrocnemius muscle of C57BL/6J mice subjected to 0- and 3-days hind limb suspension, and in biopsies from human vastus lateralis muscle obtained before and

SUBMITTER: Leermakers PA 

PROVIDER: S-EPMC6900132 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature