Measurement of Net Rate Constants from Enzyme Progress Curves without Curve Fitting.

ABSTRACT: A method is described whereby net rate constants can be directly inferred from the progress curves of enzyme intermediates without the need for model specification, numerical analysis, curve fitting, or the steady-state approximation. Specifically, if an enzyme intermediate in an ultimately irreversible serial subsequence is perturbed from and returns back to its equilibrium state as the substrate is consumed, then its net rate constant is given by the ratio of the total substrate consumed and the area under the progress curve for the enzyme intermediate. A rigorous analysis demonstrates this result to hold independent of the complete enzymatic reaction in which the subsequence is embedded, making it broadly applicable to a very wide range of kinetic mechanisms, including those complicated

SUBMITTER: Ruszczycky MW 

PROVIDER: S-EPMC6910778 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature