Human iPSC-derived microglia assume a primary microglia-like state after transplantation into the neonatal mouse brain.

ABSTRACT: Microglia are essential for maintenance of normal brain function, with dysregulation contributing to numerous neurological diseases. Protocols have been developed to derive microglia-like cells from human induced pluripotent stem cells (hiPSCs). However, primary microglia display major differences in morphology and gene expression when grown in culture, including down-regulation of signature microglial genes. Thus, in vitro differentiated microglia may not accurately represent resting primary microglia. To address this issue, we transplanted microglial precursors derived in vitro from hiPSCs into neonatal mouse brains and found that the cells acquired characteristic microglial morphology and gene expression signatures that closely resembled primary human microglia. Single-cell RNA-sequenci

SUBMITTER: Svoboda DS 

PROVIDER: S-EPMC6911218 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature