Dataset on <i>in vitro</i> maintenance of <i>Mansonella perstans</i> microfilariae and drug testing.

ABSTRACT: Endemic communities of Mansonella perstans infections have been neglected since associated pathology remains undefined. Consequently, improvements in drug therapy have also been ignored despite a large number of infected individuals in areas of Cameroon. Thus, we established an in vitro system to culture M. perstans microfilariae (Mf); the transmission stage of infection. In short, we compared the ability of two renowned culture media (Dulbecco's Modified Eagle's Medium (DMEM) and Roswell Park Memorial Institute (RPMI-1640)) to sustain Mf in culture. Media were supplemented with 10% fetal bovine serum (FBS) and monkey kidney epithelial cells (LLC-MK₂) were used as feeder cells. As readout we assessed Mf survival and motility using a standardised microscopy a

SUBMITTER: Njouendou AJ 

PROVIDER: S-EPMC6920388 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature