Dataset Information

Mandible and iliac osteoblasts exhibit different Wnt signaling responses to LMHF vibration.

ABSTRACT:

Objective

The jaw bones and long bones have distinct developmental origins and respond differently to mechanical stimuli. This study aimed to compare the Wnt signaling responses of human mandible osteoblasts and long bone osteoblasts to low-magnitude, high-frequency (LMHF) mechanical vibration in vitro.

Methods

Primary human osteoblast cultures were prepared from mandibular bone (n = 3) and iliac bone (n = 3) specimens (six individuals). Osteoblast cell lines were subjected to vibration (0, 30, 60, 90, or 120 Hz) for 30 min. After 24 h, cells were vibrated for 30 min again, then harvested immediately to quantify Wnt10b, Wnt5a and runt-related transcription factor 2 (RUNX2) mRNA expression, β-catenin protein expression and alkaline phosphatase (ALP) activity.

Results

Mandible and iliac osteoblasts responded differently to LMHF vibration: Wnt10b mRNA was upregulated by the frequency range tested; Wnt5a, β-catenin protein expression and RUNX2 mRNA expression were not altered. Furthermore, vibration upregulated ALP activity in mandible osteoblasts, but not in iliac osteoblasts.

Conclusions

This study demonstrates mandible osteoblasts and long bone osteoblasts respond differently to LMHF mechanical vibration in terms of Wnt signaling expression and ALP activity. Therefore, the effects of whole-body vibration on the long bones cannot be generalized to the jaw bones. Furthermore, osteoblast-like cells mediate the cellular responses to vibration, at least in part, by secreting extracellular signaling molecules.

SUBMITTER: Pravitharangul A

PROVIDER: S-EPMC6924195 | biostudies-literature | 2019 Oct-Dec

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Mandible and iliac osteoblasts exhibit different Wnt signaling responses to LMHF vibration.

Pravitharangul Anute A Suttapreyasri Srisurang S Leethanakul Chidchanok C

Journal of oral biology and craniofacial research 20191004 4

<h4>Objective</h4>The jaw bones and long bones have distinct developmental origins and respond differently to mechanical stimuli. This study aimed to compare the Wnt signaling responses of human mandible osteoblasts and long bone osteoblasts to low-magnitude, high-frequency (LMHF) mechanical vibration <i>in vitro</i>.<h4>Methods</h4>Primary human osteoblast cultures were prepared from mandibular bone (<i>n</i> = 3) and iliac bone (<i>n</i> = 3) specimens (six individuals). Osteoblast cell lines ...[more]

PMID: 31890493

Similar Datasets

Project description:This is the first genome-wide transcriptional profiling study using RNA-sequencing to investigate osteoblast responses to different titanium surface topographies, specifically between machined, smooth and acid-etched, microrough surfaces. Rat femoral osteoblasts were cultured on machine-smooth and acid-etched microrough titanium disks. The culture system was validated through a series of assays confirming reduced osteoblast attachment, slower proliferation, and faster differentiation on microrough surfaces. RNA-sequencing analysis of osteoblasts at an early stage of culture revealed that gene expression was highly correlated (r = 0.975) between the two topographies, but 1.38 % genes were upregulated and 0.37 % were downregulated on microrough surfaces. Upregulated transcripts were enriched for immune system, plasma membrane, response to external stimulus, and positive regulation to stimulus processes. Structural mapping confirmed microrough surface-promoted gene sharing and networking in signaling pathways and immune system/responses. Target-specific pathway analysis revealed that Rho family G-protein signaling pathways and actin genes, responsible for the formation of stress fibers, cytoplasmic projections, and focal adhesion, were upregulated on microrough surfaces without upregulation of core genes triggered by cell-to-cell interactions. Furthermore, disulfide-linked or -targeted extracellular matrix (ECM) or membranous glycoproteins such as laminin, fibronectin, CD36, and thrombospondin were highly expressed on microrough surfaces. Finally, proliferating cell nuclear antigen (PCNA) and cyclin D1, whose co-expression reduces cell proliferation, were upregulated on microrough surfaces. Thus, osteoblasts on microrough surfaces were characterized by upregulation of genes related to a wide range of functions associated with the immune system, stress/stimulus responses, proliferation control, skeletal and cytoplasmic signaling, ECM-integrin receptor interactions, and ECM-membranous glycoprotein interactions, furthering our knowledge of the surface-dependent expression of osteoblastic biomarkers on titanium.

Dataset Information

Mandible and iliac osteoblasts exhibit different Wnt signaling responses to LMHF vibration.

Objective

Methods

Results

Conclusions

Publications

Mandible and iliac osteoblasts exhibit different Wnt signaling responses to LMHF vibration.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets