Mandible and iliac osteoblasts exhibit different Wnt signaling responses to LMHF vibration.

ABSTRACT:

Objective

The jaw bones and long bones have distinct developmental origins and respond differently to mechanical stimuli. This study aimed to compare the Wnt signaling responses of human mandible osteoblasts and long bone osteoblasts to low-magnitude, high-frequency (LMHF) mechanical vibration in vitro.

Methods

Primary human osteoblast cultures were prepared from mandibular bone (n = 3) and iliac bone (n = 3) specimens (six individuals). Osteoblast cell lines were subjected to vibration (0, 30, 60, 90, or 120 Hz) for 30 min. After 24 h, cells were vibrated for 30 min again, then harvested immediately to quantify Wnt10b, Wnt5a and runt-related transcription factor 2 (RUNX2) mRNA expression, β-catenin protein expression and alkaline phosphata

SUBMITTER: Pravitharangul A 

PROVIDER: S-EPMC6924195 | biostudies-literature | 2019 Oct-Dec

REPOSITORIES: biostudies-literature