Influence of T2DM and prediabetes on blood DC subsets and function in subjects with periodontitis.

ABSTRACT:

Objective

To compare the myeloid and plasmacytoid DC counts and maturation status among subjects with/without generalized periodontitis (GP) and type 2 diabetes mellitus (T2DM).

Methods

The frequency and maturation status of myeloid and plasmacytoid blood DCs were analyzed by flow cytometry in four groups of 15 subjects: healthy controls, T2DM with generalized CP (T2DM + GP), prediabetes with GP (PD + GP), and normoglycemics with GP (NG + GP). RT-PCR was used to determine levels of Porphyromonas gingivalis in the oral biofilms and within panDCs. The role of exogenous glucose effects on differentiation and apoptosis of healthy human MoDCs was explored in vitro.

Results

Relative to controls and to NG + GP, T2DM + GP showed significantly lower CD1c + and CD303 + DC count

SUBMITTER: Rabelo MS 

PROVIDER: S-EPMC6933074 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature