Dataset Information

ZIF-8 Degrades in Cell Media, Serum, and Some-But Not All-Common Laboratory Buffers.

ABSTRACT: The emergence of drug delivery using water stable metal-organic frameworks has elicited a lot of interest in their biocompatibility. However, few studies have been conducted on their stability in common buffers, cell media, and blood proteins. For these studies, single crystal ZIF-8 approximately 1 um in diameter were synthesized, incubated with common laboratory buffers, cell media, and serum, and then characterized by PXRD, IR, DLS, and SEM. Time-resolved SEM and PXRD demonstrate that buffers containing phosphate and bicarbonate alter the appearance and composition of ZIF-8; however, cargo inside the ZIF-8 does not appear to leak out, in most of these buffers, even when the ZIF-8 itself is displaced by phosphates. On the other hand, blood proteins in serum dissolve ZIF-8, causing trapped biomolecules to escape. The study presented here suggests that ZIF-8 can undergo dramatic surface chemistry changes that may affect the interpretation of cellular uptake and cargo release data. On the other hand, it provides a rational explanation as to how ZIF-8 neatly dissolves in vivo.

SUBMITTER: Luzuriaga MA

PROVIDER: S-EPMC6938391 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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ZIF-8 Degrades in Cell Media, Serum, and Some-But Not All-Common Laboratory Buffers.

Luzuriaga Michael A MA Benjamin Candace E CE Gaertner Michael W MW Lee Hamilton H Herbert Fabian C FC Mallick Snipta S Gassensmith Jeremiah J JJ

Supramolecular chemistry 20190511 8

The emergence of drug delivery using water stable metal-organic frameworks has elicited a lot of interest in their biocompatibility. However, few studies have been conducted on their stability in common buffers, cell media, and blood proteins. For these studies, single crystal ZIF-8 approximately 1 um in diameter were synthesized, incubated with common laboratory buffers, cell media, and serum, and then characterized by PXRD, IR, DLS, and SEM. Time-resolved SEM and PXRD demonstrate that buffers ...[more]

PMID: 31892768

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Dataset Information

ZIF-8 Degrades in Cell Media, Serum, and Some-But Not All-Common Laboratory Buffers.

Publications

ZIF-8 Degrades in Cell Media, Serum, and Some-But Not All-Common Laboratory Buffers.

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