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Sheathless Shape-Based Separation of Candida Albicans Using a Viscoelastic Non-Newtonian Fluid.

ABSTRACT: Rapid and accurate identification of Candida albicans from among other candida species is critical for cost-effective treatment and antifungal drug assays. Shape is a critical biomarker indicating cell type, cell cycle, and environmental conditions; however, most microfluidic techniques have been focused only on size-based particle/cell manipulation. This study demonstrates a sheathless shape-based separation of particles/cells using a viscoelastic non-Newtonian fluid. The size of C. albicans was measured at 37 °C depending on the incubation time (0 h, 1 h, and 2 h). The effects of flow rates on the flow patterns of candida cells with different shapes were examined. Finally, 2-h-incubated candida cells with germ tube formations (≥26 μm) were separated from spherical candida cells and shorter candida cells with a separation efficiency of 80.9% and a purity of 91.2% at 50 μL/min.

SUBMITTER: Nam J

PROVIDER: S-EPMC6952941 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

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Sheathless Shape-Based Separation of Candida Albicans Using a Viscoelastic Non-Newtonian Fluid.

Nam Jeonghun J Jee Hyunseul H Jang Woong Sik WS Yoon Jung J Park Borae G BG Lee Seong Jae SJ Lim Chae Seung CS

Micromachines 20191126 12

Rapid and accurate identification of Candida albicans from among other candida species is critical for cost-effective treatment and antifungal drug assays. Shape is a critical biomarker indicating cell type, cell cycle, and environmental conditions; however, most microfluidic techniques have been focused only on size-based particle/cell manipulation. This study demonstrates a sheathless shape-based separation of particles/cells using a viscoelastic non-Newtonian fluid. The size of C. a ...[more]

PMID: 31779188

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