Homogeneous production and characterization of recombinant N-GlcNAc-protein in Pichia pastoris.

ABSTRACT: BACKGROUND:Therapeutic glycoproteins have occupied an extremely important position in the market of biopharmaceuticals. N-Glycosylation of protein drugs facilitates them to maintain optimal conformations and affect their structural stabilities, serum half-lives and biological efficiencies. Thus homogeneous N-glycoproteins with defined N-glycans are essential in their application in clinic therapeutics. However, there still remain several obstacles to acquire homogeneous N-glycans, such as the high production costs induced by the universal utilization of mammalian cell expression systems, the non-humanized N-glycan structures and the N-glycosylation microheterogeneities between batches. RESULTS:In this study, we constructed a Pichia pastoris (Komagataella phaffii) expression system producin

SUBMITTER: Wang S 

PROVIDER: S-EPMC6956495 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature