Pilose antler peptide promotes osteoblast proliferation, differentiation and mineralization via the insulin signaling pathway.

ABSTRACT: Osteoporosis is a severe bone disease characterized by a decrease in the density and structure of bones, with high risks of fractures. Pilose antler peptide (PAP), extracted and purified from deer antlers, can promote regeneration and fracture healing, and strengthen sinews and bone. To determine whether PAP can promote osteoblast development and to elucidate the molecular mechanisms underlying its functions, the present study investigated the effects of PAP on osteoblast proliferation, differentiation and mineralization, and the role of the insulin signaling pathway using MTT assay, alkaline phosphatase activity assay, western blot analysis and reverse transcription-quantitative PCR. The present results suggested that PAP promoted osteoblast proliferation, differentiation and mineralizati

SUBMITTER: Yun C 

PROVIDER: S-EPMC6966112 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature