Dataset Information

Histologic effect of the potassium-titanyl phosphorous laser on laryngeal papilloma.

ABSTRACT:

Objectives

Tissue effects occurring with potassium-titanyl phosphorous (KTP) laser treatment are difficult to quantify due to the multiple variables that affect not only the fluence (energy delivered) but also the laser-tissue interaction. This histopathologic analysis of recurrent respiratory papilloma (RRP) removed after treatment with KTP laser therapy permits correlation of histologic effect with method of laser treatment.

Methods

The histopathology of RRP resected specimens in a single patient was compared following treatment with KTP laser in contact and non-contact modes as documented with intraoperative photography and video imaging.

Results

Epithelial-sparing injury selective to the microvasculature was identified on histopathologic assessment of a specimen treated with noncontact angiolysis. Highly cauterized papillomatous epithelium without identifiable vascular structures was identified on tissue removed after treatment with the KTP laser in contact mode.

Conclusion

The histopathologic assessment of acute KTP laser effect on papilloma permits correlation between technique of application and tissue effect. Similar assessments may be helpful to modify dosimetry for individual patients requiring repeated treatment and may also assist in refining the development of existing KTP laser treatment classification systems.

Level of evidence

SUBMITTER: Eigsti RL

PROVIDER: S-EPMC6997934 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Publications

Histologic effect of the potassium-titanyl phosphorous laser on laryngeal papilloma.

Eigsti Renee L RL Bayan Semirra L SL Robinson Robert A RA Hoffman Henry T HT

Laryngoscope investigative otolaryngology 20190214 3

<h4>Objectives</h4>Tissue effects occurring with potassium-titanyl phosphorous (KTP) laser treatment are difficult to quantify due to the multiple variables that affect not only the fluence (energy delivered) but also the laser-tissue interaction. This histopathologic analysis of recurrent respiratory papilloma (RRP) removed after treatment with KTP laser therapy permits correlation of histologic effect with method of laser treatment.<h4>Methods</h4>The histopathology of RRP resected specimens i ...[more]

PMID: 32025568

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Project description:Highlights • The downregulation of miR-653-5p is involved in the progression of LP.• MSCs-derived exosomal miR-653-5p suppressed the malignant behaviors of LP cells.• The role of MSCs-derived exosomal miR-653-5p in LP relied on BZW2. Objectives Although miR-653-5p has been validated to participate in the progression of multiple types of cancer, the functional role of exosomal miR-653-5p derived from Mesenchymal Stem Cells (MSCs) in Laryngeal Papilloma (LP) has still remained elusive. Hence, this study aimed to investigate the role of MSCs-derived exosomal miR-653-5p in LP. Methods LP tissues (n = 15) and adjacent normal tissues (n = 10) were collected to examine the expression level of miR-653-5p. The expression level of miR-653-5p in LP cells and normal cells was also detected. Then, miR-653-5p was overexpressed or silenced to explore its effects on the proliferation, migration, invasion, and apoptosis of LP cells. Thereafter, the effects of exosomal miR-653-5p derived from MSCs on LP cell progression and the potential regulatory mechanism of miR-653-5p were assessed. Results It was revealed that the expression level of miR-653-5p was downregulated in LP tissues and cells. In addition, miR-653-5p suppressed the proliferation, migration, invasion, and apoptosis of LP cells. Exosomes derived from MSCs played a suppressive role in LP development and mediated the transmission of miR-653-5p to LP cells. Further exploration identified Basic leucine Zipper and W2 domains 2 (BZW2) as the target of miR-653-5p. More importantly, the rescue experiments revealed that MSCs-secreted exosomal miR-653-5p efficiently inhibited the aggressive phenotypes of LP cells, which could be significantly reversed by BZW2 overexpression in LP cells. Conclusion MSCs-derived exosomal miR-653-5p exerted inhibitory effects on LP progression through targeting BZW2, which provided a novel idea for the therapy of LP. Clinical Trial registration number chictr-ior-17011021.

Dataset Information

Histologic effect of the potassium-titanyl phosphorous laser on laryngeal papilloma.

Objectives

Methods

Results

Conclusion

Level of evidence

Publications

Histologic effect of the potassium-titanyl phosphorous laser on laryngeal papilloma.

Similar Datasets

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